8B5V
Crystal Structure of Trypanosoma Cruzi phosphomannosemutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96859 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.398, 83.427, 152.131 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.550 - 1.600 |
| R-factor | 0.2007 |
| Rwork | 0.199 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f9r |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.720 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.550 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.159 | 0.663 |
| Number of reflections | 63539 | 2464 |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 92.1 | 73.6 |
| Redundancy | 4.8 | 3.1 |
| CC(1/2) | 0.976 | 0.612 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.4 | 293 | 0.1M SPG pH 8.4, 24% PEG1500 |
