8B55

Human ADGRG4 PTX-like domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	113.117, 40.243, 54.912
Unit cell angles	90.00, 113.90, 90.00

Refinement procedure

Resolution	51.710 - 1.360
R-factor	0.1571
Rwork	0.154
R-free	0.18880
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.046
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.710	1.380
High resolution limit [Å]	1.356	1.360
Rmeas	0.028	0.530
Rpim	0.011	0.230
Number of reflections	43935	282
<I/σ(I)>	29.6	2.9
Completeness [%]	89.7	20
Redundancy	6.9
CC(1/2)	0.999	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292.15	0.1 M Tris pH 8.5, 30 % PEG 4000, 0.2 M magnesium chloride