Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-04 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.402, 87.312, 37.482 |
| Unit cell angles | 90.00, 104.81, 90.00 |
Refinement procedure
| Resolution | 43.656 - 1.496 |
| Rwork | 0.149 |
| R-free | 0.18090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zud |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.848 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.310 | 1.520 |
| High resolution limit [Å] | 1.496 | 1.500 |
| Number of reflections | 34235 | 1426 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 99.3 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.996 | 0.509 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.5, 20m M MgCl2 and 22 %(w/v) polyacrylic acid 5100 sodium salt |
