8B3W

Structure of metacyclic VSG (mVSG) 1954 from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-17
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.9198
Spacegroup name	P 3 2 1
Unit cell lengths	69.614, 69.614, 115.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.288 - 1.681
R-factor	0.1736
Rwork	0.172
R-free	0.20900
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.022
Phasing software	PHENIX (1.19-4092-000)
Refinement software	PHENIX (1.19-4092-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.290	1.741
High resolution limit [Å]	1.681	1.681
Number of reflections	70911	3705
<I/σ(I)>	7.45
Completeness [%]	99.8
Redundancy	19.3
CC(1/2)	0.999	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 1500, 0.4M Sodium Bromide