8B3B

Structure of metacyclic VSG (mVSG) 531 from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	50.107, 170.885, 163.652
Unit cell angles	90.00, 90.71, 90.00

Refinement procedure

Resolution	59.090 - 1.949
R-factor	0.202
Rwork	0.200
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ly9
RMSD bond length	0.004
RMSD bond angle	0.733
Refinement software	PHENIX (1.19-4092-0000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.090	2.019
High resolution limit [Å]	1.949	1.949
Number of reflections	195472	18665
<I/σ(I)>	4.24
Completeness [%]	97.7
Redundancy	3
CC(1/2)	0.977	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 1500, 0.1M MMT buffer pH 6.5, 3% glucose