8B2Y

Structure of the weakly red fluorescent protein csiFP4 from Clytia simplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-26
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 65
Unit cell lengths	182.904, 182.904, 155.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	91.450 - 2.830
R-factor	0.212
Rwork	0.211
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hpw
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.450	3.070
High resolution limit [Å]	2.830	2.830
Rpim	0.150	0.600
Number of reflections	56144	2808
<I/σ(I)>	4	1.5
Completeness [%]	94.4
Redundancy	7.6
CC(1/2)	0.970	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1M lithium sulfate, 0.1M TRIS pH 8.5 and 0.01M nickel chloride.