8B28

Structure of an intron-retention variant of the plant immune signalling protein EDS1 from Vitis vinifera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.915079
Spacegroup name	C 1 2 1
Unit cell lengths	144.549, 65.077, 45.454
Unit cell angles	90.00, 105.48, 90.00

Refinement procedure

Resolution	43.810 - 1.750
R-factor	0.1755
Rwork	0.174
R-free	0.20590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i8g
RMSD bond length	0.003
RMSD bond angle	0.567
Data reduction software	XDS (Jan 10, 2022 (BUILT 20220820))
Data scaling software	autoPROC (1.0.5)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.810	1.942
High resolution limit [Å]	1.750	1.755
Rmerge	0.106	0.668
Number of reflections	29029	1451
<I/σ(I)>	5.7	1.4
Completeness [%]	71.3
Redundancy	2.8
CC(1/2)	0.988	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	Protein solution: 4.5 mg/ml in 300 mM sodium iodide, 1 % glycerol, 1 mM DTT, 50 mM Hepes buffer, pH 8.0). Reservoir solution: 12 % PEG3350, 300 millimolar CsCl, 100 millimolar Bis-Tris puffer, pH 8.5. Crystallization drop before equilibration: 1.5 mikroliter protein solution plus and 1.5 mikroliter reservoir solution.