8B0R

Structure of the CalpL/cA4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97626
Spacegroup name	P 32 2 1
Unit cell lengths	69.487, 69.487, 256.288
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.470 - 2.200
R-factor	0.2378
Rwork	0.236
R-free	0.26710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qda
RMSD bond length	0.002
RMSD bond angle	0.423
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20_4444)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.470	2.279
High resolution limit [Å]	2.200	2.200
Rmeas	0.049
Number of reflections	37616	3685
<I/σ(I)>	8.37
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.999	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-Cl pH 8.0, 38.8 % PEG 400, 0.29 M Li2SO4