8B0Q

Deinococcus radiodurans UvrC C-terminal half

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	50.432, 40.807, 59.636
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.810 - 1.800
R-factor	0.1682
Rwork	0.166
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nrt
RMSD bond length	0.020
RMSD bond angle	1.846
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0085)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.810	40.810	1.900
High resolution limit [Å]	1.800	5.690	1.800
Rmerge	0.065	0.038	0.310
Rmeas	0.076	0.044	0.364
Rpim	0.037	0.022	0.186
Total number of observations	89441	2908	11622
Number of reflections	22631	764	3180
<I/σ(I)>	13.7	26.3	4
Completeness [%]	99.6	98.9	98.2
Redundancy	4	3.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	24-26% PEG 5000 MME or PEG 3350 and 0.1M Bis-Tris pH 6.5