8AZG

Crystal structure of MreB from Geobacillus stearothermophilus ATCC7953 in complex with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-11-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 21 21 2
Unit cell lengths	57.740, 169.100, 43.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.470 - 2.290
R-factor	0.209
Rwork	0.207
R-free	0.25010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7zpt
RMSD bond length	0.008
RMSD bond angle	0.895
Data reduction software	XDS (1.20_4459)
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.880	2.430
High resolution limit [Å]	2.290	2.290
Rmeas	0.146	1.410
Number of reflections	19996	3130
<I/σ(I)>	12.41
Completeness [%]	99.7
Redundancy	13.13
CC(1/2)	1.000	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 8000, glycerol, potassium phosphate, magnesium chloride