8AYU

Crystal structure of SUDV VP40 L117A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000009
Spacegroup name	C 1 2 1
Unit cell lengths	62.464, 90.700, 48.380
Unit cell angles	90.00, 95.05, 90.00

Refinement procedure

Resolution	48.190 - 2.000
R-factor	0.1712
Rwork	0.170
R-free	0.19500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ld8
RMSD bond length	0.007
RMSD bond angle	0.858
Data reduction software	XDS
Data scaling software	BIOMOL
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.190	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	17832	1328
<I/σ(I)>	11.5	4.9
Completeness [%]	98.3	99.3
Redundancy	3.8	3.9
CC(1/2)	0.996	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	90 mM HEPES 6.8% (v/v) ethylen glycol 15% (v/v) glycerol 17% (v/v) PEG10,000