8AYI
Scalindua brodae amxFabZ H48N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000001 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 105.160, 105.160, 79.399 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.580 - 2.200 |
| R-factor | 0.2494 |
| Rwork | 0.248 |
| R-free | 0.26120 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1u1z |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.387 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHENIX (1.18.2_3874) |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.582 | 2.039 |
| High resolution limit [Å] | 1.955 | 1.955 |
| Rmerge | 0.059 | 1.437 |
| Number of reflections | 11349 | 568 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 93.0 | 43.6 |
| Redundancy | 6.7 | 6.2 |
| CC(1/2) | 0.997 | 0.271 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.0 M Sodium formate |
