8AYD

Anammox-specific FabZ from the annamox bacterium Kuenenia stuttgartiensis

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	P 43 2 2
Unit cell lengths	75.350, 75.350, 179.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.900 - 2.800
R-factor	0.2536
Rwork	0.252
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uiz
RMSD bond length	0.004
RMSD bond angle	0.977
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.089	0.833
Number of reflections	13435	1223
<I/σ(I)>	19
Completeness [%]	100.0
Redundancy	12.5
CC(1/2)	0.999	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.3 M ammonium sulfate