8AYB

anammox-specific FabZ from Scalindua brodae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.033217
Spacegroup name	H 3 2
Unit cell lengths	104.640, 104.640, 82.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.660 - 1.800
R-factor	0.1855
Rwork	0.185
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uiz
RMSD bond length	0.006
RMSD bond angle	0.862
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.048	0.351
Number of reflections	15501	2237
<I/σ(I)>	19	3.2
Completeness [%]	96.1
Redundancy	9.1
CC(1/2)	0.999	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	3.6 M sodium formate