8AXJ

Crystal structure of the N-terminal domain of Trypanosoma brucei CFAP410

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-06
Detector	PSI PILATUS 6M
Wavelength(s)	0.97242
Spacegroup name	P 21 21 21
Unit cell lengths	39.799, 52.960, 61.433
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 - 1.000
R-factor	0.1512
Rwork	0.151
R-free	0.16230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l4p
RMSD bond length	0.010
RMSD bond angle	1.221
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	1.040
High resolution limit [Å]	1.000	1.000
Number of reflections	69889	5835
<I/σ(I)>	10.84
Completeness [%]	98.2	83.75
Redundancy	11.4
CC(1/2)	0.998	0.386

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	293	0.1 M sodium citrate, 1 M ammonium dihydrogen phosphate