8AXG
Crystal structure of Fusobacterium nucleatum fusolisin protease
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 41 |
| Unit cell lengths | 115.475, 115.475, 196.786 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.893 - 2.040 |
| Rwork | 0.177 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | assembly |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.375 |
| Data reduction software | DIALS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.420 | 2.070 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 149218 | 4433 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 91.6 | |
| Redundancy | 12.9 | |
| CC(1/2) | 0.976 | 0.193 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 100mM Bis-Tris pH 6.5 and 20% PEG MME5000 |
