8AXF

Crystal structure of FMV N bound to 42-mer ssRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.072
Spacegroup name	P 21 21 21
Unit cell lengths	53.170, 151.820, 169.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.490 - 2.540
R-factor	0.2227
Rwork	0.222
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8ax4
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.490	48.490	2.600
High resolution limit [Å]	2.540	20.000	2.540
Rmerge	0.120	0.046	1.591
Rmeas	0.130	0.053	1.718
Total number of observations	335806
Number of reflections	45871	119	2652
<I/σ(I)>	13.34	33.44	1.17
Completeness [%]	99.1	92.2	87.6
Redundancy	7.321	4.95	6.665
CC(1/2)	0.997	0.997	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	289	20% PEG 3350 0.2 M Mg-formate