8AWF
Xylose Isomerase in 80% relative humidity environment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2022-02-16 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.100, 98.750, 94.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.780 - 1.610 |
| R-factor | 0.1799 |
| Rwork | 0.179 |
| R-free | 0.20170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rnf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.976 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.780 | 1.668 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Number of reflections | 1055859 | 10024 |
| <I/σ(I)> | 9.99 | |
| Completeness [%] | 99.0 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.991 | 0.313 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 293 | 100 mM HEPES 200 mM Lithium Sulphate 34% Peg3350 |
