8AUD

Structure of peptidoglycan hydrolase Cg1735 from Corynebacterium glutamicum, orthorhombic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98112
Spacegroup name	P 21 21 21
Unit cell lengths	96.572, 123.083, 209.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.540 - 4.500
R-factor	0.2797
Rwork	0.278
R-free	0.30710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8auc
RMSD bond length	0.005
RMSD bond angle	1.069
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.540	5.030
High resolution limit [Å]	4.500	4.500
Rpim	0.117	0.342
Number of reflections	15441	4293
<I/σ(I)>	5.5	2.3
Completeness [%]	100.0	100
Redundancy	13.5	13.7
CC(1/2)	0.992	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	2.4 M (NH4)2SO4, 0.1 M Na3 citrate.