8AUC

Structure of peptidoglycan hydrolase Cg1735 from Corynebacterium glutamicum, trigonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.07188
Spacegroup name	H 3 2
Unit cell lengths	230.580, 230.580, 416.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.570 - 3.500
R-factor	0.2278
Rwork	0.226
R-free	0.26020
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	3.580
High resolution limit [Å]	3.500	3.500
Rpim	0.049	7.886
Number of reflections	54157	3903
<I/σ(I)>	13.4
Completeness [%]	99.6	98.1
Redundancy	115.6	101.4
CC(1/2)	0.999	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	1 M (NH4)2H Citrate, 0.1 M Na Acetate pH 4.6