Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 65 |
| Unit cell lengths | 57.490, 57.490, 48.033 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.788 - 2.117 |
| Rwork | 0.196 |
| R-free | 0.25170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f44 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.488 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.259 | 1.420 |
| Rpim | 0.059 | 0.327 |
| Number of reflections | 5201 | 516 |
| <I/σ(I)> | 10.07 | 1.61 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.8 | 19.7 |
| CC(1/2) | 0.996 | 0.568 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1M Bis Tris pH5.5, 0.3M of Magnesium formate |
