8ATI

Human CtBP2(31-364) in complex with RAI2 peptide(315-322)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	291
Detector technology	PIXEL
Collection date	2018-12-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976243
Spacegroup name	P 61 2 2
Unit cell lengths	126.646, 126.646, 357.598
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.869 - 2.600
Rwork	0.228
R-free	0.28330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ome
RMSD bond length	0.008
RMSD bond angle	1.779
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.037	0.428
Rmeas	0.052	0.605
Number of reflections	53115	5227
<I/σ(I)>	12.87	1.8
Completeness [%]	99.9	100
Redundancy	2
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277.15	20% PEG 6000, 100 mM Tris pH 7.5