8ATC

COMPLEX OF N-PHOSPHONACETYL-L-ASPARTATE WITH ASPARTATE CARBAMOYLTRANSFERASE. X-RAY REFINEMENT, ANALYSIS OF CONFORMATIONAL CHANGES AND CATALYTIC AND ALLOSTERIC MECHANISMS

Experimental procedure

Spacegroup name	P 3 2 1
Unit cell lengths	122.110, 122.110, 156.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.165
RMSD bond length	0.014 *
RMSD bond angle	0.037 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	2.400 *
High resolution limit [Å]	2.300 *
Total number of observations	415000 *
Number of reflections	51500 *
Completeness [%]	84.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	dialyze *		21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein solution	10-18 (mg/ml)
2	1	2	maleic acid	50 (mM)
3	1	2	N-ethylmorpholine	50 (mM)
4	1	2	PALA	1 (mM)
5	1	2		3 (mM)