8ASR
The Crystal structure of F46Y mutant of apo agroavidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID09 |
| Synchrotron site | ESRF |
| Beamline | ID09 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-02 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.322, 84.781, 67.915 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.760 - 1.660 |
| R-factor | 0.18033 |
| Rwork | 0.179 |
| R-free | 0.21467 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ew1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.693 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.760 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.778 | |
| Number of reflections | 53732 | 5477 |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 98.6 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.990 | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 18% pentaerythritol ethoxylate, 0.05 M Bis Tris pH 5.9-6.0, 0.05 M ammonium sulphate |
