8ART
ABC transporter binding protein MalE from Streptomyces scabiei in complex with maltose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.310, 68.310, 260.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.890 - 3.170 |
| R-factor | 0.262 |
| Rwork | 0.260 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Alphafold model |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.790 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.893 | 3.360 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Rmerge | 0.175 | 2.724 |
| Number of reflections | 12628 | 1917 |
| <I/σ(I)> | 12.63 | |
| Completeness [%] | 99.4 | |
| Redundancy | 19 | |
| CC(1/2) | 0.999 | 0.379 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 200mM ammonium acetate, 100mM sodium acetate pH 4.6 and 30% PEG 4000 |
