8ARD

Myosin VI proximal tail domain, dimeric

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-27
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.978570
Spacegroup name	P 65 2 2
Unit cell lengths	29.387, 29.387, 295.649
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.270 - 2.219
R-factor	0.3243
Rwork	0.323
R-free	0.33810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	30x Ser helix
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	XDS (20180409)
Data scaling software	STARANISO (1.10.15)
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (8-JUN-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.270	2.458
High resolution limit [Å]	2.219	2.219
Rmerge	0.168	2.414
Rmeas	0.174	2.506
Number of reflections	2977	199
<I/σ(I)>	11.7	1.3
Completeness [%]	66.9	55.9
Redundancy	18.2	14
CC(1/2)	0.997	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	290	27% PEG 4000, 10 mM MgCl2, 0.2 M imidazole-malate, pH 6.0