Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-06 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 1.3776 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 78.847, 78.847, 110.981 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.277 - 1.900 |
| Rwork | 0.195 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yqq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.424 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.280 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.106 | |
| Number of reflections | 28350 | 1798 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 23.2 | |
| CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | Bis-Tris Propane [20mM] pH7.0, Glycerol [20%], PEG 8000 [16%], Monopotassium phosphate [40mM] |
