8AON
Oxidoreductase fragment of human QSOX1 in complex with a Fab fragment of a humanized anti-QSOX1 antibody
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2022-02-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.168, 98.721, 63.090 |
| Unit cell angles | 90.00, 101.19, 90.00 |
Refinement procedure
| Resolution | 49.360 - 2.100 |
| R-factor | 0.2039 |
| Rwork | 0.198 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ij3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.908 |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.360 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.105 | |
| Rpim | 0.075 | 1.127 |
| Number of reflections | 102949 | 3817 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 97.0 | 98.55 |
| Redundancy | 2.7 | 2.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, ammonium phosphate dibasic |
