8ANX

E329A Mutant Thermogutta terrifontis endoglucanase catalytic domain with C-linker from glycoside hydrolase family 5 (TtEnd5A-CDC-E329A)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-17
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	60.390, 69.590, 81.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.590 - 1.200
Rwork	0.120
R-free	0.14840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8ag9
RMSD bond length	0.008
RMSD bond angle	1.436
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.590	1.160
High resolution limit [Å]	1.130	1.130
Rmerge	0.045	1.646
Rmeas	0.047
Rpim	0.013
Number of reflections	125321	7072
<I/σ(I)>	18.9
Completeness [%]	96.8	75.2
Redundancy	11.6	4.6
CC(1/2)	1.000	0.392

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2M NH4Cl and 20%W/v PEG 3350