8ANR
Fucosylated alternate chirality linear peptide FHP30 bound to the fucose binding lectin LecB PA-IIL from Pseudomonas aeruginosa at 1.6 Angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-13 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000023 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 70.691, 70.691, 103.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 1.620 |
| R-factor | 0.15 |
| Rwork | 0.148 |
| R-free | 0.18340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oxc |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.626 |
| High resolution limit [Å] | 1.618 | 1.618 |
| Rmeas | 0.152 | |
| Number of reflections | 63963 | 10328 |
| <I/σ(I)> | 15.46 | 0.98 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13.85 | 13.43 |
| CC(1/2) | 0.999 | 0.378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2 M Ammonium citrate tribasic pH 7.0, 20% polyethylene glycol 3350 |
