8ANM
Structure of the amyloid-forming peptide LYIQWL from Tc5b, grown from water
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-14 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 9.492, 21.675, 23.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.990 - 0.900 |
| R-factor | 0.1314 |
| Rwork | 0.130 |
| R-free | 0.14510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ideal 5 residue beta strand form the software Fragon |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.155 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | Fragon |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.990 | 0.930 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmeas | 0.128 | 0.672 |
| Number of reflections | 3952 | 392 |
| <I/σ(I)> | 9.1 | 2.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.57 | |
| CC(1/2) | 0.999 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 310 | Peptide was dissolved in water in 0.15 mg/ml concentration followed by pH adjustment with NaOH to pH 4.0 - 6.0. Crystals grew after incubation at 310K. |
