8ANJ
Structure of the amyloid-forming peptide DFINWL from human GLP-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-09 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 21.780, 4.869, 21.936 |
| Unit cell angles | 90.00, 101.73, 90.00 |
Refinement procedure
| Resolution | 16.950 - 1.550 |
| R-factor | 0.1689 |
| Rwork | 0.168 |
| R-free | 0.17440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | crystal structure of LYIQWL |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.956 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.960 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmeas | 0.067 | 0.356 |
| Number of reflections | 773 | 77 |
| <I/σ(I)> | 12.1 | 2.2 |
| Completeness [%] | 98.3 | 87.5 |
| Redundancy | 2.88 | |
| CC(1/2) | 0.999 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 310 | Lyophilized peptide was dissolved in 0.6 mg/ml concentration in a solution containing 30 % acetonitrile and 0.1 % TFA and incubated at 310K for 4 weeks. |
