8ANG
Structure of the amyloid-forming peptide LYIQWL from Tc5b, grown from 30% ethanol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-21 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 4.875, 23.713, 40.764 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.500 - 1.500 |
| R-factor | 0.0852 |
| Rwork | 0.084 |
| R-free | 0.09530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ideal 5 residue beta strand form the software Fragon |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.236 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | Fragon |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.500 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.066 | 0.204 |
| Number of reflections | 895 | 61 |
| <I/σ(I)> | 13.5 | 3.8 |
| Completeness [%] | 95.0 | 65.59 |
| Redundancy | 4.06 | |
| CC(1/2) | 0.998 | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 293 | Peptide was dissolved in 30% ethanol at 0.5 mg/ml concentration, then incubated at 293K overnight. |
