8AN0

Endo-D-arabinofuranase AraH2 from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	H 3 2
Unit cell lengths	145.674, 145.674, 92.681
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.500 - 2.410
R-factor	0.2151
Rwork	0.213
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	alphafold model
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.500	2.450
High resolution limit [Å]	2.410	2.410
Number of reflections	153262	715
<I/σ(I)>	5.4
Completeness [%]	100.0
Redundancy	10.4
CC(1/2)	0.986	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.8M Succinic acid pH 7