8AMV
RepB pMV158 hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.910, 79.330, 114.900 |
| Unit cell angles | 90.00, 104.92, 90.00 |
Refinement procedure
| Resolution | 45.480 - 2.770 |
| R-factor | 0.2206 |
| Rwork | 0.218 |
| R-free | 0.26710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dkx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.410 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.520 | 45.520 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.083 | 1.097 |
| Number of reflections | 40217 | 3702 |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 99.2 | |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 100 mM Na/K phosphate pH 6.2 200 mM NaCl 10 % PEG 8000 |
