8AMU
RepB pMV158 OBD domain bound to DDR region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.234, 33.619, 289.672 |
| Unit cell angles | 90.00, 93.51, 90.00 |
Refinement procedure
| Resolution | 24.690 - 3.000 |
| R-factor | 0.2298 |
| Rwork | 0.227 |
| R-free | 0.28180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dkx |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.814 |
| Data reduction software | autoPROC |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (1.20rc4_4425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.700 | 24.700 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.164 | 0.530 |
| Number of reflections | 17954 | 1433 |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 90.5 | |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50 mM Tris-HCl pH 8.0 50 mM MgCl2 23 % PEG3350 |
