8AMM
Crystal structure of AUGUGGCAU duplex with cesium ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.917143 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.231, 90.612, 139.933 |
Unit cell angles | 90.00, 91.43, 90.00 |
Refinement procedure
Resolution | 45.310 - 2.860 |
R-factor | 0.2238 |
Rwork | 0.221 |
R-free | 0.26880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8amg |
RMSD bond length | 0.002 |
RMSD bond angle | 0.465 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.310 | 3.030 |
High resolution limit [Å] | 2.860 | 2.860 |
Rmerge | 0.080 | 1.586 |
Number of reflections | 30808 | 4633 |
<I/σ(I)> | 8.51 | 0.81 |
Completeness [%] | 96.0 | |
Redundancy | 3.5 | |
CC(1/2) | 0.999 | 0.527 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | 0.08 M NaCl 0.02 M CsCl 0.04 M cacodylate sodium 45% MPD 0.012 M spermine |