8AM4

Cl-rsEGFP2 Long Wavelength Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I23
Synchrotron site	Diamond
Beamline	I23
Temperature [K]	50
Detector technology	PIXEL
Collection date	2021-07-08
Detector	DECTRIS PILATUS 12M
Wavelength(s)	3.0996
Spacegroup name	P 21 21 21
Unit cell lengths	50.915, 63.250, 70.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.110 - 2.020
Rwork	0.196
R-free	0.25720
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.110	2.080
High resolution limit [Å]	2.020	2.020
Rmerge	0.032	0.112
Rmeas	0.046	0.159
Rpim	0.032	0.112
Number of reflections	12297	571
<I/σ(I)>	24.7
Completeness [%]	80.7
Redundancy	1.9
CC(1/2)	0.998	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM Hepes pH 8.0, 1.80 M ammonium sulphate, 20 mM NaCl
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM Hepes pH 8.0, 1.80 M ammonium sulphate, 20 mM NaCl