8ALU

Crystal structure of the teichoic acid binding domain of SlpA, S-layer protein from Lactobacillus acidophilus (aa. 314-444)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.95
Spacegroup name	C 1 2 1
Unit cell lengths	80.430, 27.050, 61.890
Unit cell angles	90.00, 91.05, 90.00

Refinement procedure

Resolution	40.210 - 2.090
R-factor	0.2133
Rwork	0.208
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qeh
RMSD bond length	0.002
RMSD bond angle	0.398
Data reduction software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.210	2.170
High resolution limit [Å]	2.090	2.090
Rmerge	0.046	0.555
Rmeas	0.056	0.700
Rpim	0.031	0.418
Number of reflections	12330	340
<I/σ(I)>	15.46	1.53
Completeness [%]	84.5	44
Redundancy	2.9	2.3
CC(1/2)	0.999	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Index screen condition #80 (0.2 M ammonium acetate, 0.1 M HEPES pH 7.5 and 25 % PEG 3350) with a protein stock solution of 20 mg/mL in 20 mM ADA pH 6.5 and 100 mM NaCl.