8ALH

X-ray structure of human NCS-1 bound to Ric-8A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979263
Spacegroup name	P 41 21 2
Unit cell lengths	56.861, 56.861, 134.612
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.380 - 1.860
R-factor	0.1975
Rwork	0.196
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qi4
RMSD bond length	0.009
RMSD bond angle	0.920
Data reduction software	AutoProcess (1.0.5)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.380	1.942
High resolution limit [Å]	1.854	1.854
Number of reflections	18029	881
<I/σ(I)>	15.2
Completeness [%]	94.0
Redundancy	8.7
CC(1/2)	0.999	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	30 % PEG 4000, 100 mM sodium acetate pH 4.6, 100 mM magnesium chloride