8AK3

Drosophila melanogaster UNC89 Protein Kinase 1 in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9795
Spacegroup name	P 63 2 2
Unit cell lengths	95.418, 95.418, 164.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.160 - 1.900
R-factor	0.1752
Rwork	0.173
R-free	0.20780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8ak2
RMSD bond length	0.008
RMSD bond angle	1.053
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.160	1.920
High resolution limit [Å]	1.900	1.900
Number of reflections	35642	1080
<I/σ(I)>	14.83
Completeness [%]	100.0
Redundancy	12.53
CC(1/2)	0.998	0.312

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	291.15	1M potassium sodium tartrate, 100 mM CHES pH 9.5, 200 mM lithium sulfate