8AJ7

Kunitz domain of Amblyomin-X

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	26.540, 46.090, 76.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.430 - 1.600
R-factor	0.258
Rwork	0.256
R-free	0.29750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y62
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.430	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	22828	1578
<I/σ(I)>	8.7
Completeness [%]	96.2
Redundancy	14.9
CC(1/2)	0.990	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein: 9 mg/ml. Precipitant: 50% (36% PEG 600, 0.1 HEPES pH 7.5) and 50% (36% PEG 2000, 0.1 sodium cacodylate pH 6.5)