8AJ4
X-ray structure of lysozyme obtained upon reaction with [VIVO(malt)2] (Structure A')
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.080, 77.080, 38.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.540 - 1.220 |
| Rwork | 0.178 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.813 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.540 | 1.240 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Rmerge | 0.090 | 1.005 |
| Number of reflections | 34981 | 1663 |
| <I/σ(I)> | 19.1 | 3.1 |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 18.6 | 12.4 |
| CC(1/2) | 0.999 | 0.747 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2.0 M sodium formate 0.1 M hepes pH 7.5 |
