8AIP
Crystal Structure of Two-domain bacterial laccase from the actinobacterium Streptomyces carpinensis VKM Ac-1300
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2020-01-30 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 97.962, 97.962, 97.962 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.650 - 2.350 |
| R-factor | 0.2337 |
| Rwork | 0.231 |
| R-free | 0.28140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gyb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.957 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 | 0.355 |
| Number of reflections | 13826 | 1147 |
| <I/σ(I)> | 6.8 | 1.5 |
| Completeness [%] | 97.0 | 82.3 |
| Redundancy | 4.29 | |
| CC(1/2) | 0.990 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 20%v/v PEG Smear Broad, 0.1 M Sodium citratate, pH 5.6, 0.15 M Mg acetate (condition #33 of BCS-1 from Molecular Dimensions, UK) |
