8AIK

Crystal structure of DltE from L. plantarum, tartare bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	48.690, 103.730, 152.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.384 - 1.950
Rwork	0.182
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8agr
RMSD bond length	0.007
RMSD bond angle	1.433
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.384	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.050	0.564
Number of reflections	56946	3918
<I/σ(I)>	14.5	2.4
Completeness [%]	99.5
Redundancy	4.5
CC(1/2)	0.999	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, ammonium tartrate