8AIC

X-ray structure of the receptor binding domain of Env glycoprotein of Simian Foamy virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	123.624, 123.624, 191.611
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.730 - 2.800
R-factor	0.1961
Rwork	0.194
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Related deposition D_1292124233
RMSD bond length	0.004
RMSD bond angle	0.761
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.730	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.199	1.705
Rpim	0.044	0.372
Number of reflections	40619	4041
<I/σ(I)>	13.35
Completeness [%]	99.6	99.14
Redundancy	21.6	21.6
CC(1/2)	0.997	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M ammonium tartarate, 20% w/v PEG 3350