8AHD
The apo structure of the Corramycin phosphotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-03 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.088, 69.863, 117.536 |
| Unit cell angles | 90.00, 98.11, 90.00 |
Refinement procedure
| Resolution | 59.900 - 2.100 |
| R-factor | 0.2109 |
| Rwork | 0.208 |
| R-free | 0.26710 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.400 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.100 | 0.520 |
| Number of reflections | 45561 | 6647 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 97.1 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | Magnesium chloride, Tris, PEG 8000 |
