8AGY

The Corramycin phosphotransferase in complex with Corramycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	42.964, 67.116, 75.553
Unit cell angles	90.00, 93.33, 90.00

Refinement procedure

Resolution	42.890 - 1.500
R-factor	0.1755
Rwork	0.175
R-free	0.19370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Apo structure
RMSD bond length	0.006
RMSD bond angle	1.004
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.890	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.062	0.442
Number of reflections	67775	9808
<I/σ(I)>	22.7
Completeness [%]	99.8
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG 10000, HEPES