8AFI

GABARAP in complex with LIR motif of HsATG3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-27
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	62.800, 63.800, 73.900
Unit cell angles	64.50, 86.50, 70.30

Refinement procedure

Resolution	49.100 - 2.660
R-factor	0.217
Rwork	0.215
R-free	0.26180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hb9
RMSD bond length	0.011
RMSD bond angle	1.216
Data reduction software	XDS (1.16_3549)
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.130	2.750
High resolution limit [Å]	2.660	2.660
Rmerge	0.068	1.126
Rmeas	0.081
Rpim	0.043	0.721
Number of reflections	52724	2663
<I/σ(I)>	14.19	1.02
Completeness [%]	98.1
Redundancy	3.5
CC(1/2)	0.998	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Sodium acetate, sodium cacodylate, PEG 8,000