8AEL

SYNJ2BP complex with a synthetic Vangl2 peptide (3mer).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-04
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	59.670, 75.060, 108.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.530 - 2.200
R-factor	0.2235
Rwork	0.221
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jik
Data scaling software	XSCALE
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.530	37.530	2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.298	0.083	1.187
Rmeas	0.309	0.086	1.231
Total number of observations	340828
Number of reflections	24694	341	1834
<I/σ(I)>	6	18.09	0.99
Completeness [%]	96.6	96.3	99.1
Redundancy	13.802	11.745	14.225
CC(1/2)	0.993	0.998	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Ammonium Sulfate, 0.1 M Sodium formate, 0.1 M HEPES 7.0, 25% v/v PEG Smear Broad